Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. Phusion DNA Polymerase possesses the following activities: 53 DNA polymerase activity and 35 exonuclease activity. Includes: Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/L) 5X Phusion HF Buffer. $164.00 - $652.00 Specifications Concentration 2 U/L View More Specs Includes: Phusion High-Fidelity DNA Polymerase If reactions are set up according to recommendations listed, the final concentration of Phusion DNA Polymerase in the reaction is 1 unit/50 l or 0.4 units/20 l. of Reactions: 500: Polymerase: Phusion High . Place the reaction tubes on ice and add to each tube 10x RT buffer 2 l RT enzyme mix 2 l RNase-free H 2 O 6 l 5. Thermo Scientific's Phusion Plus DNA Polymerase is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Thermo Fisher phusion polymerase enzyme Phusion Polymerase Enzyme, supplied by Thermo Fisher, used in various techniques. 50X higher fidelity than Taq. Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. Learn more.Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Store at -20C. Phusion is a registered trademark and property of Thermo Fisher Scientific. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. PCR amplificaton of target plasmid with two phosphorylated primers. DMSO. 2. Thermo Scientific Phusion High-Fidelity DNA Polymerase (2 U/L) Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. F549L $665.00 / Each of 1 Qty Check Availability Add to cart Phusion DNA Polymerase Concentration: The concentration of Phusion DNA Polymerase in the Phusion PCR Master Mix has been optimized for best results under a wide range of conditions. cDNA obtained from total RNA isolated from HEF 293 Flp-In T-REx cells was used as a template to amplify the ORF coding for the full-length human DXO wild type by PCR with the primer pair hDXO1For-hDXO1Rev (0.2 M both) and using Phusion High-Fidelity DNA Polymerase (F530, Thermo Fisher Scientific) with 1 HF buffer, 0.2 mM dNTP mix and 3% DMSO. Phusion was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific . Hot Start II DNA Polymerase is equal to that of Phusion DNA Polymerase (4.4 10-7 in Phusion HF buffer) when determined with a modified lacI-based method1. 500 U (2 U/L) 3900.00 NOK. Thermo Scientific Phusion Blood Direct PCR Master Mix Pub. F530L. phusion hs ii polymerase ( Thermo Fisher ) 97 Thermo Fisher phusion hs ii polymerase Phusion Hs Ii Polymerase, supplied by Thermo Fisher, used in various techniques. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. HF Buffer Pack. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Reagents Supplied. Select input method Single pair Batch 3. Introduction. GC Buffer Pack. Select your DNA polymerase Platinum SuperFi DNA polymerase (Also select this option if using the SuperScript IV One-Step RT-PCR Kit) Phusion or Phire DNA polymerase DreamTaq DNA polymerase or other Taq -based DNA polymerase 2. Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Phusion DNA Polymerase, 400 M of each dNTP and 2X Phusion HF Buffer; DMSO; Phusion High-Fidelity PCR Master Mix with GC Buffer (F-532): Phusion DNA Polymerase, 400 M of each dNTP and 2X Phusion GC Buffer; DMSO; Master mixes provide 1.5 mM MgCl 2 and 200 M dNTP in final reaction concentration. Phusion Hot Start II High-Fidelity DNA Polymerase is capable of amplifying long amplicons such as the 7.5 kb genomic and 20 kb DNA used in Thermo Fisher Scientific quality control assays. F530S $119.34 / Each Qty Add to cart Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mix of Phusion Plus DNA Polymerase, buffer, magnesium chloride and dNTPs. Phusion High-Fidelity DNA Polymerase For high speed and high performance PCR. Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Thermo Scientific Phusion High-Fidelity DNA Polymerase Phusion DNA Pub. Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. Thermo Scientific F530S Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Bioz Stars score: 97/100, based on 63 PubMed citations. of Reactions: 100: Polymerase: Phusion High . Phusion DNA Polymerase (2 U/L) 5X Phusion HF Buffer 5X Phusion GC Buffer DMSO 50 mM MgCl 2 solution Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl 2 in the final 1X concentration. Phusion Blood II DNA This kit uses the highly processive Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase for exponential PCR amplification of dsDNA plasmid to be mutated. The high fidelity makes the PhusionHigh-Fidelity DNA Polymerase a superior choice for cloning. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Phusion. Phusion DNA Polymerase (2U/l) is provided with 5x Phusion HF Buffer and 5x Phusion GC Buffer. https://www.bioz.com/result/phusion high fidelity dna polymerase/product/Thermo Fisher Average 99 stars, based on 1 article reviews Price from $9.99 to $1999.99 phusion high fidelity dna polymerase - by Bioz Stars , 2022-08 99 / 100 stars Buy from Supplier t4 ligase ( Thermo Fisher ) 98 Thermo Fisher t4 ligase It also produced superior yields of Incorporating an exciting new technology, Phusion. Use 15-30 s/kb for extension. Applications Incubate at 65C for 5 minutes to predenature the RNA. ZERO BIAS - scores, article reviews, protocol conditions and more The enzyme, originally developed by Finnzymes, is a fusion of a proofreading polymerase and a processivity-enhancing domain, creating one of the most reliable and robust polymerases available. Choose Phusion Polymerase from Thermo for highly accurate, fast and robust PCR. Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. 50X higher fidelity than Taq Robust Reactions - maximal success with minimal optimization Kit includes everything needed for a PCR reaction, including controls Kit Components Thermo Scientific F553L Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS: 200 x 50L Rxns (500 x 20L Rxns) In addition, its specialized reaction buffer allows PCR amplification without the need to calculate primer annealing temperatures, helping you save time and avoid mistakes in PCR runs. Specifications. High fidelity provides >100X higher . Phusion is a registered trademark and property of Thermo Fisher Scientific. High fidelity provides >100X higher sequence accuracy than Taq polymerase Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA . Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. B.00) . Phusion Hot Start II High Fidelity DNA Polymerase is the most accurate hot start DNA polymerase on the market. Brand: Thermo Scientific F-530XL Additional Details : Weight : 1.00000kg . Add to cart. 500 units. No. 5X Phusion GC Buffer. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. 4. Phusion high-fidelity DNA polymerases The family of Phusion high-fidelity DNA polymerases displays low error rates during PCR and includes over a dozen various formats. View alternative products. Hot start formulation reduces non-specific amplification. High fidelity provides >100X higher sequence accuracy than Taq polymerase This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion DNA Polymerase generates long templates with an accuracy and speed . Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA . Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Phusion High-Fidelity PCR Kit (F-553): Phusion . Phusion DNA Polymerase exploits this dramatic increase in processivity, resulting in shorter extension times, more robust and high yield amplification, and the ability to amplify long templates in a fraction of the time. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Phusion DNA polymerase 0.5 L 0.2 L 0.02 U/L Thermo Scientific Phusion High-Fidelity DNA Polymerases offer very high fidelity, speed, and yield for all PCR applications. 1. A.00) Extension . Since their introduction, Thermo Scientific Phusion High-Fidelity DNA Polymerases have been referenced in thousands of publications for high-performance PCR and have become the choice for a multitude of demanding applications ranging from reconstruction, design and massively-parallel, high-throughput sequencing of whole genomes. Manufacturer: Thermo Scientific F530L View more versions of this product Catalog No. https://www.bioz.com/result/phusion hotstartii high fidelity dna polymerase/product/Thermo Fisher Average 98 stars, based on 1 article reviews Price from $9.99 to $1999.99 phusion hotstartii high fidelity dna polymerase - by Bioz Stars , 2022-09 98 / 100 stars Buy from Supplier Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. For Phusion DNA Polymerase (Thermo Fisher) Standard 5X Phusion HF buffer * - 10 ul 98C - 1 min 10mM dNTP - 1 ul 98C - 15 s Primers/each (10M) - 2.5 ul 55C - 20 s 30 cycles cDNA OR - 0.5 ul 72C -* Genomic DNA OR - 0.5 ul 72C - 4 min Plasmid DNA - 1.0 ul 4C - for ever . Source Thermo Scientific Phusion DNA Polymerases set a gold standard for PCR performance. Thermo Scienti c Phusion high- delity DNA polymerases have been referenced in thousands of publications and have become . Both buffers contain 1.5 mM MgCl 2 at final reaction concentrations. Offered with multiple buffers for customized reaction setup for different DNA templates. Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final 1X concentration.Store at -20C. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. The Phusion High-Fidelity DNA Polymerase brings together a Pyrococcus-like enzyme with a processivity-enhancing domain. Phusion Hot Start II High-Fidelity The colored buffer does not interfere with excelent performance . Master mix format for ease of use and optimized performance. Pyrococcus furiosus. MAN0012393 details Rev. ZERO BIAS - scores, article reviews, protocol conditions and more ZERO BIAS - scores, article reviews, protocol conditions and more Phusion Plus Buffer 1.25 mL 5 1.25 mL 20 1.25 mL Phusion GC Enhancer 1.25 mL 4 1.25 mL 16 1.25 mL General guidelines Use 98C for denaturation. Phusion DNA Polymerase Concentration: We generally recommend using Phusion DNA Polymerase at a concentration of 20 units/ml (1.0 units/50 l reaction). Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. 100 U (2 U/L) Phusion is a registered trademark and property of Thermo Fisher Scientific. instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA . It is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Component Amount Storage Phusion Hot Start II DNA Polymerase, 2 U/L 10 L 20C 5X Phusion HF Buffer 1.5 mL dNTP Mix 10 mM each 20 L FastDigest DpnI 20 L New Phusion Green format is a combination of Phusion DNA Polymerase and 5X Phusion Green Buffers. General instructions Due to the unique nature of Phusion DNA polymerases, always use the T m . Use 200 M of each dNTP. However, the optimal concentration of Phusion DNA Polymerase may vary from 10-40 units/ml (0.5-2 units/50 l reaction) depending on amplicon length and difficulty. Create optimal reaction conditions for high fidelity amplification. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the . PG: Phusion Green High-Fidelity DNA Polymerase P: Phusion High-Fidelity DNA Polymerase This protocol describes the method used for the amplification of specific DNA fragments by the Polymerase Chain Reaction, using plasmid DNA as a template. Thermo Scientific Phusion High-Fidelity DNA Polymerase offers extreme performance for all major PCR applications. As a hot-start, proofreading PCR enzyme, Phusion Plus DNA polymerase enables generation of PCR amplicons with very high sequence accuracy, sensitivity, and specificity. 50X higher fidelity than Taq Robust reactions - maximal success with minimal optimization 100 U (2 U/L) Each for $164.00. Program a thermal cycler as outlined in Table 1. Following purification, these can be used in downstream assays, including . 50X higher fidelity than Taq. MAN0016449 Rev. Phusion Polymerase: robust and accurate high-fidelity PCR. Manufacturer: Thermo Scientific F549L View more versions of this product Catalog No. Thermo Scientific Phusion High-Fidelity DNA Polymerase (2 U/L) Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. 50X higher fidelity compared to Taq Robust reactions - maximal success with minimal optimization Master mix format with HF buffer ideal for a wide range of AT/GC content Phusion Flash DNA Polymerase is free of contaminating endo- and exonucleases.The polymerase is capable of amplifying long amplicons such as the 7.5 kb genomic and 20 kb lambda DNA used in Thermo Scientific's quality control assays. Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. The mutagenesis protocol comprises four steps: 1. DNA polymerase. Phusion. 50 mM MgCl2 solution. Its unique structure, a novel Pyrococcus -like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Thermo Scientific Bovine Genotypes Panel 3.1 Kit is offered for cattle parentage testing and identification using Short . They offer a combination of characteristics that no other enzyme can match. PhusionU DNA Polymerase carries a mutation in the uracil- binding pocket to overcome this limitation. Separate tubes of DMSO and 50 mM MgCl 2 solutions are provided for further optimization. DNA polymerase. Since their introduction, Thermo Scientific Phusion High-Fidelity DNA Polymerases have been referenced in thousands of publications for high- performance. Type or paste your sequence 4. Click here to acc ess our Tm calculator. Do not use dUTP (The polymerase cannot read through dUTP-derivatives or dITP in the template strand, Thermo Scientifi cPhusionU Hot Start DNA Polymerase retains all features of Phusion family enzymesgreat accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specifi city with Affi body ligand-based hot start. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus . Date 09 January 2017 (Rev. Finnzymes' Phusion High-Fidelity DNA Polymerase offers extreme performance for all PCR applications. Phusion Blood II DNA Polymerase is a proofreading polymerase that contains a unique processivity-enhancing domain, making this polymerase accurate and rapid. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. The PhusionHigh- Fidelity DNA Polymerase generates long amplicons with accuracy and speed, even on the most difficult templates. No. Fidelity (vs. Taq) 52 X: GC-Rich PCR Performance: High: No. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. This product is related to the following categories: Reaction Buffers, Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Create optimal reaction conditions for high fidelity amplification. Thermo Scientific's Phusion Plus DNA Polymerase is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. This unique protein fusion technology enables Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. It generates blunt ends in the amplification products. Thermo Scientific Phusion RT-PCR Kit Product Information Store at -20C 3. The amplified products may subsequently be analysed for presence or absence and size in agarose gels. a conventional Taq DNA polymerase. High-Fidelity PCR Master Mix containing Phusion Flash II DNA Polymerase was able to amplify the 1.5 kb gene with very short extension times of 10 and 20 seconds. Description Thermo Scientific's Phusion Plus DNA Polymerase is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs. Store at -20C. High fidelityprovides >100X higher sequence accuracy than Taq polymerase Phusion DNA Polymerase (1 U) Superior yields of long PCR products A 20 kb fragment from DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion DNA Polymerases and proofreading DNA polymerases from other suppliers.